A quiet request from Pbob, suffering from paperptosis. Call it what you like! I am writing a review of the use of flow cytometry analysis in truly rare cell enumeration. I am not looking at analysis only and not sorting, except where it's used essentially for verification of the counting, eg to ensure that the counted cell was actually the target cell. I define truly rare cell enumeration as counting cells occurring at frequencies at or below one "target" cell per thousand abundant cells. I might consider one per 100, but presently I prefer not to, as I consider 1% pretty trivial. I am open to argument though. When I was a mainstream academic back in the 1980s, people such as Jim Leary, Jan Visser, Paul Horan, Ken Bauer, Jim Cupp and Betsy Ohlsson-Wilhelm were the pace-setters in these areas. I guess that there are others who have now joined them in this area, especially given the advances in foetal cell and stem cell research, including the recent revelations about CNS stem cells. If you have written or know of a paper where flow measurements were used to count such cells within my definition, would you please let me know about it. Attached files are welcome! I prefer that you answer off-List, but should anyone want a copy of the resultant paper-list, I will forward it upon request. Probably about the end of the month, when my son finishes rowing season! I plan to cover as wide an area of usage as exists out there, whether it be transfection studies in eukaryotes or prokaryotes, bacterial mutants, leukocytes in packed red cells, foetal bleeding, minimum residual disease, diagnosis of metastasis, developmental-cell trafficking, monitoring treatment regimes, clinical or fundamental, medical or biological, etc, etc. Whatever. It will cover use of extrinsic and intrinsic markers, a variety of laser excitations and the benefits of multi-colour-epitope labelling vs single fluorochromes, doublet discrimination, acquisition rates, sensitivity etc. I am wildly out of touch with the Journal Literature, but I hope that y'all out there can assist. Thanks for your time and possible input. Pbob
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