you wrotte, >I have a client who would like to attach pasturella to fluorescent beads >in order to measure pasturella specific phagocytosis. Has anyone out there >done this? >If you have, would you be willing to share your information. We would be >interested in, what fluorescent beads you used, what protocol you used to >attach the beads to the pasturella, etc. Any and all info on this >subject >will be appreciated. > Thanks in advance, > Joan K > Flow Cytometry Lab Supervisor > VMRCVM > (540) 231-4115 > FAX 540-231-7367 > jkalnits@vt.edu I am actually working on a similar project. But like Arnold Pizzey, I find quite usefull to use fluorescein diacetate. I can also label your pasturella with PKH-26 (Molecular probes) and follow endocytosis. If you need more details tell me. Good luck. Amicalement Patrice Dr. Petit Patrice X. Institut Cochin de Génétique Moléculaire INSERM U129 - CHU Cochin Port-Royal 24, rue du Faubourg Saint-Jacques F-75014 Paris, France. Tel: 33 01 44 41 24 11 Fax: 33 01 44 41 24 21 E-mail: pxpetit@icgm.cochin.inserm.fr
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