>I have a client who would like to attach pasturella to fluorescent beads >in order to measure pasturella specific phagocytosis. Has anyone out there >done this? >If you have, would you be willing to share your information. We would be >interested in, what fluorescent beads you used, what protocol you used to >attach the beads to the pasturella, etc. Any and all info on this >subject >will be appreciated. > Thanks in advance, > Joan K > > > Flow Cytometry Lab Supervisor > VMRCVM > (540) 231-4115 > FAX 540-231-7367 > jkalnits@vt.edu Greetings Joan, A few years ago I had a quick look at neutrophil phagocytosis of candida sp. by simply growing up the yeast in medium containing fluorecein diacetate overnight, then washing them and then feeding them to neuts. It seemed to work - gating on the neutrophil cloud and observing the 525 emmision. I don't know if this would work for pasturella but it might be worth a quick go. Best wishes Arnold. -------------------------------- Arnold Richard Pizzey Department of Haematology 98 Chenies Mews London WC1E 6HX a.pizzey@ucl.ac.uk (044) 0171 209 6234 fax: 0171 209 6222 -------------------------------
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