Altig,Kathy wrote: > > We are planning to perform Ki-67 on paraffin preserved tissue by flow. > This will replace the ploidy analysis, as it seems to be out of favor. > I am trying to find the best or most practical method and antibody. > Suggestions? > > Thank you, > Kathy Altig > SW Washington Med. Ctr. Dear Kathy, When you use the Ki-S5 antibody from Boehringer Mannheim, then you can quantify the Ki67-positive cells in cell suspensions prepared from paraffin embedded tissues after trypsin digestion step. Ki-S5 is an antibody also directed against a formalin resistant epitope of Ki67. Mathie Leers Atrium Medical Centre Heerlen Dept. of Pathology P.O.Box 4446 6401 CX Heerlen The Netherlands
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:05 EST