Jeffrey, you may use U937 cells from ATCC as FcR positive cell line. The variant we use in our lab originally was obtained from ATCC and was found to be contaminated with another line some years later(!?). After FACS sorting we got the cell line (U937-KR) which is CD64+ and CD32+ but CD16-. Best regard Georg Kreysch ------------------------------------------------------------------------ H.G. Kreysch Merck KGaA Biomed Fo/IMO - A25/410 Tel.: (49)6151 - 72 7323 Frankfurter Str. 250 FAX: (49)6151 - 72 7398 D-64271 Darmstadt E-Mail: kreysch@merck.de
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