Retroviral vectors

From: Rice, Susan E. (Fka Grigsb (srice1@iupui.edu)
Date: Tue Jan 26 1999 - 12:35:30 EST


Dear Flow Group,

Could someone please bring me up to speed on sorting human cells infected
with retrovirus which are used for research purposes?  I understand that
these reagents are supposed to be defective viruses used for genetic
transfer.  What biohazard classification has a laboratory working with these
vectors?  What precautions over and above the common practices are required?
Will bleaching through the sample probe between sorts eliminate viruses from
the flow cytometer to prevent viral contamination of subsequent sorts?

Please cite references if available.  Can someone cite a reference that
lists the requirements for laboratories according to the different biohazard
classifications?

Thanks in advance,
Sue Rice
 



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