The NIH recombinant guidelines indicates the mouse retroviral vectors can be used at BSL-1. The quote is as follows: Appendix B-V-1. Murine Retroviral Vectors Murine retroviral vectors to be used for human transfer experiments (less than 10 liters) that contain less than 50% of their respective parental viral genome and that have been demonstrated to be free of detectable replication competent retrovirus can be maintained, handled, and administered, under BL1 containment. The URL is: http://www.orcbs.msu.edu/biological/NIH/appendixb.htm > -----Original Message----- > From: Rice, Susan E. (Fka Grigsb [SMTP:srice1@iupui.edu] > Sent: Tuesday, January 26, 1999 12:36 PM > To: Cytometry Mailing List > Subject: Retroviral vectors > > > Dear Flow Group, > > Could someone please bring me up to speed on sorting human cells infected > with retrovirus which are used for research purposes? I understand that > these reagents are supposed to be defective viruses used for genetic > transfer. What biohazard classification has a laboratory working with > these > vectors? What precautions over and above the common practices are > required? > Will bleaching through the sample probe between sorts eliminate viruses > from > the flow cytometer to prevent viral contamination of subsequent sorts? > > Please cite references if available. Can someone cite a reference that > lists the requirements for laboratories according to the different > biohazard > classifications? > > Thanks in advance, > Sue Rice >
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