I am in the process of setting up a flow cytometric method for measuring lymphocyte subset activation and could use some help. We have a FACSCalibur and are interested in using PHA, pokeweed and Con-A as mitogens; we are also considering using CD2/CD2R as a stimulant. We are planning on assessing expression of CD69, CD3, CD4, CD8 and CD19. Our main issues involve: 1) stimulation protocols, 2) optimal mitogen stimulation times and 3) potential differences between adult and pediatric populations. If anyone could either share their experiences and/or suggest a good reference to help us with this process, it would be much appreciated. Thank you, Yvonne G. Dowdy, M.D.
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:52:59 EST