4 color staining and Argon laser

From: robert gniadecki (rgniadecki@hotmail.com)
Date: Fri Jan 22 1999 - 01:57:29 EST


Hello!
We are planning immunophenotyping with 4 colours: FITC, PE, Pe-Texas red 
(ECD) and Pe-Cy5. We have however experienced some severe problems with 
compensation between PE and PE-TR.

Could you share your experience with me? Is that fluorochrome 
combination possible to use?

We have 4 photomultipliers and a 488 nm argon laser.
Thanks, 

Robert Gniadecki
Bispebjerg hospital
Copenhagen, Denmark

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