Re: 4 color staining and Argon laser

From: Rachel M. Gerstein (Rachel.Gerstein@ummed.edu)
Date: Fri Jan 29 1999 - 08:35:06 EST


You could use Cy7-PE (using bandpass filter 785+/-50) in the place of
PE-TR. Caltag and Pharmingen both sell some Cy7-PE reagents.  see also
Mario Roederer's site:  http://cmgm.stanford.edu/~roederer/abcon/.

Rachel M. Gerstein, Ph.D.
Asst. Professor
Dept. of Mol. Gen. & Micro.
University Of Massachusetts Medical School
55 Lake Avenue North
Worcester, MA 01655
508-856-1044 (phone)
508-856-5920 (FAX)


robert gniadecki wrote:

> Hello!
> We are planning immunophenotyping with 4 colours: FITC, PE, Pe-Texas red
> (ECD) and Pe-Cy5. We have however experienced some severe problems with
> compensation between PE and PE-TR.
>
> Could you share your experience with me? Is that fluorochrome
> combination possible to use?
>
> We have 4 photomultipliers and a 488 nm argon laser.
> Thanks,
>
> Robert Gniadecki
> Bispebjerg hospital
> Copenhagen, Denmark
>
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