Hello flowers, I repeat my question as I did not get the original mail from Wednesday and maybe, this mail was lost. Excuse me, if you get this mail a second time! A colleague want to make cell fusions. For this purposes, the membane of one cell population (B-cells) should be labelled for later analysis of the fusion by flow cytometry. I proposed pkH-26 from Sigma for membrane labeling, and in addition, the labeling of the DNA of the second cell population with Hoechst 33342. Cells from positive fusions should have blue nuclei and red membranes (suitable for the analysis by flow cytometry). Is this choice acceptable or does anybody knows better alternatives? We would be happy for any references. Thanks Andreas PD Dr. Andreas Simm Institut für klinische Biochemie und Pathobiochemie Versbacher Str. 5 97078 Würzburg Tel.: 0931-201-3479 FAX.: 0931-201-3153
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