Nuclei doublets would fall in this region. Saverio Alberti Head, Unit of Experimental Oncology Department of Cell Biology and Oncology Consorzio Mario Negri Sud 66030 Santa Maria Imbaro (Chieti), Italy Phone: (39) 872-570.293 FAX: (39) 872-578.240 E-mail: alberti@cmns.mnegri.it On Mon, 11 Jan 1999, Robert Walt St. George Fisher wrote: > > Hi. I'm somewhat of a beginner at flow cytometry, so I'd like to post a > question to the group. I have noticed in some of my samples a subpopulation > of cells that I can't figure out. I'm doing a PI vs. BrdU incorporation > analysis on whole, EtOH fixed epithelial cells, and the population in question > shows up in the DNA area vs. DNA width diagram. It has 4N DNA content, but > increased DNA width so it runs above the G2/M population in this diagram. > Would senescent cells show up in this area, since they tend to get larger and > flatten out? If you gate this subpopulation to a DNA area vs. BrdU fluorescence > diagram, they have the same BrdU fluorescence as G2/M cells. > > Go to this URL to see what I'm talking about: > http://www.radonc.unc.edu/~fisher/flow.gif > > I'm open to suggestions as to what this population represents.... > > Thank you. > Robert Fisher > fisher@radonc.unc.edu > >
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