Hi. I'm somewhat of a beginner at flow cytometry, so I'd like to post a question to the group. I have noticed in some of my samples a subpopulation of cells that I can't figure out. I'm doing a PI vs. BrdU incorporation analysis on whole, EtOH fixed epithelial cells, and the population in question shows up in the DNA area vs. DNA width diagram. It has 4N DNA content, but increased DNA width so it runs above the G2/M population in this diagram. Would senescent cells show up in this area, since they tend to get larger and flatten out? If you gate this subpopulation to a DNA area vs. BrdU fluorescence diagram, they have the same BrdU fluorescence as G2/M cells. Go to this URL to see what I'm talking about: http://www.radonc.unc.edu/~fisher/flow.gif I'm open to suggestions as to what this population represents.... Thank you. Robert Fisher fisher@radonc.unc.edu
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