Tim Kute asked: > Dear all DNA analysis people, > > Different calculation methods for flow cytometric S-phase fraction: > prognostic implications in breast cancer? > Bo Baldetorp et al Cytometry 33 385-393,1998 ( Dec 1 issue) > > > Tim Kute > Dr. Timothy Kute Telephone # = 336-716-2950 > Associate Professor in Pathology email = tkute@bgsm.edu > Wake Forest University School of Medicine > Medical Center Blvd > Winston-Salem, NC 27157-1072 ---------------------- Tim, I certainly think this was a valuable study and hope that the oncologists see it. > A recent article was published in Cytometry on S phase analysis and prognosis in breast cancer. The title of the article and reference are listed below: > I would be interested in hearing comments on this paper. I will try to summarize the comments after a two week period. I believe that this paper has much "food for thought". Happy new year. The article that you mentioned is very interesting. Based on these results we should all be doing S phase analysis and the values are significant for prognosis of breast cancer. I agree that when comparing between patients, one must use the same lab and each lab should establish its' own tertiles for prognosis. I found the information on background subtraction very interesting. This mathematical manipulation seems to be unnecessary and may actually introduce additional variability in the assay. This makes sense, since the settings for subtraction are highly subjective. However, I did not see a discussion of the effect of variable background between different preparations from patients with the same type of tumor. It seems that this would also complicate comparison and that is why we did the subtraction originally. Best regards, Tony Bakke Antony C. Bakke, Ph.D. Director, Special Immunology and Flow Cytometry Oregon Health Sciences University Portland, OR (503) 494-4687
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:52:55 EST