Do the antibody incubations in 5 % serum from the species of animal the antibodies were generated in. The antibodies in the serum should block the non-specific binding. On Fri, 8 Jan 1999, Dr. Alwina Bittner wrote: > > Hi collegues, > please accept me as a new member of the FACS-User-Group. > My first question: Has anyone experience in staining mouse splenocytes? I > have a problem with the high unspecific binding of several antibodies. Does > anybody know a method how to overcome this Problem. > > Thanks in advance > > Alwina > Dr. Alwina Bittner > MZ Nervenheilkunde - Neurologische Klinik mit Poliklinik > Neuroimmunologisches Labor > Rudolf-Bultmann-Str.8, 35039 Marburg, Germany, > Fax +49 6421 28 7055, Phone +49 6421 28 5480 >
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