Hello Alwina Have you tried using an anti-Fc (anti-CD16/32) antibody (Pharmingen sells it as"Fc-Block"). This has helped increase the separation from negative and positive peaks for some stains. And, using BSA or Serum in your staining buffer can help. (but then, you probably know about the serum) :-) Artur Plett On Fri, 8 Jan 1999, Dr. Alwina Bittner wrote: > > Hi collegues, > please accept me as a new member of the FACS-User-Group. > My first question: Has anyone experience in staining mouse splenocytes? I > have a problem with the high unspecific binding of several antibodies. Does > anybody know a method how to overcome this Problem. > > Thanks in advance > > Alwina > Dr. Alwina Bittner > MZ Nervenheilkunde - Neurologische Klinik mit Poliklinik > Neuroimmunologisches Labor > Rudolf-Bultmann-Str.8, 35039 Marburg, Germany, > Fax +49 6421 28 7055, Phone +49 6421 28 5480 >
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