I am sorry, I am having problems with my server and many of my replies are delivered with no sense phrases. Here we are again: I work with mouse spleenocytes, PBL, LNL and BM. I always had unspecific binding before I start to blok this undesired reactions with PBS 3% FBS and also with 1% of serum form the source of my monoclonal. Good luck. Olindo
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