RE: new member - unspecific antibody binding to mouse splenocytes

From: Olindo Assis Martins Filho (oamfilho@netra.cpqrr.fiocruz.br)
Date: Tue Jan 12 1999 - 05:58:45 EST


I am sorry, I am having problems with my server and many of my replies are delivered with no sense phrases. Here we are again:

I work with mouse spleenocytes, PBL, LNL and BM. I always had unspecific
binding before I start to blok this undesired reactions with PBS 3% FBS
and also with 1% of serum form the source of my monoclonal.  Good luck.

Olindo



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