> Can anyone point me to references where intracellular > cytokine staining by flow cytometry was compared > directly to assays (e.g. ELISA) of secreted cytokine or > of cytokine message? What I am interested in is whether > there is any data correlating frequencies of cytokine > producing cells with total secreted cytokine or cytokine > mRNA levels. Look at Baumgarth, N., Egerton, M. & Kelso, A. Activated T cells from draining lymph nodes and an effector site differ in their responses to TCR stimulation. J. Immunol. 159:1182-91 (1997). This paper didn't do ELISA, but did do bio-assays. In addition, we have done extensive comparison of cytokine profiles by mRNA and by FACS in human T cell subsets, finding good correlation--hopefully will be published very soon. In addition to the frequency of producers being a correlate of production by the whole population, the relative fluorescence within cells appears to correlate (grossly) with their production. However, I wouldn't rely on this latter correlation for anything better than order-of-magnitude. mr
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:51:44 EST