Hi all Can anyone point me to references where intracellular cytokine staining by flow cytometry was compared directly to assays (e.g. ELISA) of secreted cytokine or of cytokine message? What I am interested in is whether there is any data correlating frequencies of cytokine producing cells with total secreted cytokine or cytokine mRNA levels. I know work relating mRNA levels to secreted cytokine, but not intracellular cytokine to secreted cytokine Thanks for your help. Margaret A. Cooley School of Microbiology and Immunology The University of New South Wales Sydney, NSW 2052 Australia Phone:61-2-9385-3761 Fax: 61-2-9385-1591
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