Try these: Jung T, Schauer U, Heusser C, Neumann C, Rieger C: Detection of intracellular cytokines by flow cytometry. J. Immunol. Met. 150:197-207, 1993. Kabilan L, Anderson G, Lolli F, Ekre H-P, Olsson T, Troye-Blomberg M: Detection of intracellular expression and secretion of interferon-gamma at the single-cell level after activation of human T cells with tetanus toxoid in vitro. Eur. J. Immunol. 20:1085-1089,1990. One problem with intracellular staining seems to be the fact that you can't distinguish cytokine that is produced by the cell from cytokine that is internalized by the cell. In addition, just because a cell is a high producer, that doesn't necessarily mean it is a high secretor. Prussin and Metcalfe have also shown that there is an "extracellular pool" of cytokine that can be labelled without permeablising the cells. See: Prussin C and Metcalfe DD: Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J. Immunol. Met. 188:117-128, 1995. Happy Flowing! David McFarland Howard Hughes Medical Institute Flow Cytometry Facility Vanderbilt University Medical Center _______________________________________________________________________________ Subject: Intracellular cytokine staining versus secreted cytokine From: Margaret Cooley <m.cooley@unsw.EDU.AU> at +inet Date: 02/02/1998 2:24 PM Hi all Can anyone point me to references where intracellular cytokine staining by flow cytometry was compared directly to assays (e.g. ELISA) of secreted cytokine or of cytokine message? What I am interested in is whether there is any data correlating frequencies of cytokine producing cells with total secreted cytokine or cytokine mRNA levels. I know work relating mRNA levels to secreted cytokine, but not intracellular cytokine to secreted cytokine Thanks for your help. Margaret A. Cooley School of Microbiology and Immunology The University of New South Wales Sydney, NSW 2052 Australia Phone:61-2-9385-3761 Fax: 61-2-9385-1591
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:51:44 EST