chromosome sorting - again

From: Dennis J. Young (djyoung@UCSD.Edu)
Date: Tue Jan 27 1998 - 12:21:02 EST


The question has come up again - Can one sort chromosomes with low-power
Argon laser(s)
(ONE water cooled & tunable, one 488 nm ONLY) in a FACStar+?

1) single laser 488 nm  TOTO-1 (AT selective) and 7-AAD (GC selective)?

2) single laser UV (350/360 nm)?

3) 457 nm and 488 nm dual lasers?

4) 350/360 nm and 488 nm dual lasers?


Dennis

*************************************************************************
* Dennis J. Young                            Voice : (619) 822-0407     *
* Flow Cytometry Core Facility               FAX   : (619) 822-0412     *
* University of California, San Diego  USA   e-mail: djyoung@ucsd.edu   *
*                       http://www-core.ucsd.edu/flow-core.html         *
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