Dear Colleagues, Recently I tried to view Cy5 in the Fl4 channel of my FACSTAR Plus and had considerable difficulty getting a decent signal beyond the first log, while that same sample gave a signal beyond the third log on the Calibur. I'm wondering if there is any significant reason why I shouldn't be able to duplicate HeNe laser 'conditions' on the FACSTAR Plus using Rhodamine 6G in a Dye Head. I was even using approximately the same bandpass, a 660/30? Any thoughts? Gene
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