Heterokaryon analysis by FACS

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I am looking for expertise for performing heterokaryon analysis by FACS.  The 
overall goal is to examine the effect of cell fusion on the conformation of a 
cell surface molecule, 'X'.  Cell types 'A' and 'B' can be uniquely 
distinguished by antibodies to cell surface molecules, and the conformation of X 
can also be distinguished by reactivity with a panel of antibodies.  I would 
think one could fuse A with B using PEG, then identify heterokaryons and 
determine the conformation of X all using FACS.

Anyone know of any references to this type of experiment, or personal experience 
that can help me think through the potential problems with analysis?

Please respond by email:

Chan Beals MD PhD
cbeals@icos.com 

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