Baboon bone marrow ...

From: huw kruger gray (zip@biotransplant.com)
Date: Tue Jan 20 1998 - 13:27:41 EST

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Greetings! 

    We have a group here who are trying to analyse baboon bone marrow for the
presence (or not) of transfected DNA, which is carrying a marker gene for GFP.
The problem which they have encountered is that these baboon bone marrow cells
have an enormously high apparent green auto-fluorescence (?) level, which makes
accurate detection & quantification of the transduced GFP fluorescence extremely
difficult. Is there anyone out there (!) who is, or has, worked with flow
cytometric analysis of baboon bone marrow &/or who has encountered a similar
situation ... ??? 

Thanks, 

Huw S. ("Zip") Kruger Gray, Ph.D. 
           >>>---> 
BioTransplant Inc. 
(617)-241-5200 x 254.

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