At 12:16 15.01.98 -0800, you wrote: > > >Dear Flow Folks: > >does anybody have practical suggestions about assessment of purity of >recovered cells after magnetic (positive) sort using Dynal beads? > >Beads are coated with rat anti mouse isotype-specific Ab. >I have problem in detaching beads from cells (incomplete detachment ), in >beads intrinsic fluorescence (when beads are run without staining as >control at the FACS) and in cross-reactivity between the GAM that I am >suppose to use to quantify the purity (according to the manufacturer >protocol) and the RAM. > Most of you may know them... I would recommend using smaller magnetic prticles to sort your cells. Miltenyi Biotech sells them as MiniMACS reagents. They are compatible with FACS and work great. There is no need to detach those particles to reanalyze sorted populations, but if there is need to detach there also exist detachable particles try their website: http://www.miltenyibiotec.com/ hp ******************************************* hpsteff@mzdmza.zdv.uni-mainz.de Hans-Peter Steffens http://vzdmzi.zdv.uni-mainz.de/~hpsteff/virology.html Institute for Virology, Johannes Gutenberg-University Mainz Obere Zahlbacher Strasse 67, 55101 MAINZ, Germany Tel.: ++49-6131-17-3165 Fax: ++49-6131-39-5604 *******************************************
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