Greetings to all! I have a question about rat lymphocytes that maybe one of you out there can answer.......... I am trying to purify rat B cells by negative selection with monoclonals (I need them to be unstimulated) and by Percoll gradients. The Percoll procedure that I have is for human or mouse B cells. Rat cells are of a different (lighter) specific gravity than these cells and the percents of Percoll listed in my protocol (85, 75 and 50 with the B cells to band between the 85 and 75% layers) do not work. Anyone have any experience in this area? or does anyone know what specific gravity rat small B cells are? Thanks Lynne Anguish **************************************** Lynne J. Anguish Rm 106 James A. Baker Institute Hungerford Hill Rd. Cornell University, Ithaca,NY 14853 (607)256-5610 FAX (607)256-5608 e-mail lja2@cornell.edu ****************************************
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