Dear Flow Folks: does anybody have practical suggestions about assessment of purity of recovered cells after magnetic (positive) sort using Dynal beads? Beads are coated with rat anti mouse isotype-specific Ab. I have problem in detaching beads from cells (incomplete detachment ), in beads intrinsic fluorescence (when beads are run without staining as control at the FACS) and in cross-reactivity between the GAM that I am suppose to use to quantify the purity (according to the manufacturer protocol) and the RAM. I will really appreciate all your ideas. Thanks in advance for the help Mara ------------------------------------------- Dr. Mara Rocchi, DVM Ph.D., Lab 4, International Livestock Research Institute Nairobi, Kenya Tel: 254-2-630743 ext. 4403 Fax: 254-2-631499 e-mail: M.Rocchi@cgnet.com
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