Dear MAK, We developed a first prototype for quantitation of TNFR I and TNFR II on WBC in whole blood, as well as on cultured cells. The results we obtained are as follows (approximative numbers, I am not in my office and this is memory recollection): Results on normal individuals: TNFR I: approx. 600 molecules /lymphocyte. Same on granulocytes. TNFR II: 2,800 molecules/granulocyte. On lymphocytes, we observed two sub populations. The first population gives about 100 molecules/lymphocyte. The second population gives about 2,000 molecules/ lymphocyte. This looks exactly like what we observed for the Fas receptor quantitation on lymphocytes using the APOCYT Fas kit (ref to my E-mail on this list / 12-22-97). Coming back to the TNFR I and II receptors, these results imply the use of a calibrator allowing quantitation of very low expressions. Best regards Michel M. Canton, PharmD Managing Director, BioCytex France Tel: +33 (0) 4 91 94 29 39 Fax: +33 (0) 4 91 47 24 71 >Hello, >I have interest in quantifying TNF receptors on cells by flow. Given the >availability of standards for MESF determination with MOAb binding (Flow >Standards, BioCytex), has anyone done similar analyses for growth factors, >or anything comparable? Any thoughts will be appreciated. >MAK. >--- >Mark A. KuKuruga, Managing Director >University of Michigan Flow Cytometry >kukuruga@medmail.med.umich.edu > >
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