Complement Activation (Forwarded message)

From: J.Paul Robinson (jpr@flowcyt.cyto.purdue.edu)
Date: Tue Jan 13 1998 - 23:21:48 EST


I am posting this request. Please reply directly to
stoutej@AfricaOnline.co.ke  with answers:


----------------------------------
>Dear all:
>Any help on the following question would be greatly appreciated:
>
>I am interested in detecting complement deposition on red cell surfaces.
>Can anybody
>what would be the best way to collect blood such that the complement
>cascade will not be activated (what types of tubes, anticoagulant, etc)
>and after collection how should the samples be stored so as to avoid
>complement cascade activation (room temperature vs. 4oC?) and for how
>long would they be good?
>
>Thank you,
>
>
>--
>    Jose A. Stoute, MD
>    Unit 64109,  Box 401
>    USAMRU-Kenya
>    APO AE 09831-4109 Tel 254-2-729303,  Fax 254-2-714592
>    stoutej@AfricaOnline.co.ke

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Message posted by:








J.Paul Robinson, Ph.D.
Professor of Immunopharmacology
Director, Purdue University Cytometry Laboratories
EMAIL: robinson@flowcyt.cyto.purdue.edu
WEB http://www.cyto.purdue.edu
Phone: (765) 494 0757  FAX (765) 494 0517



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