If I recall, the PAC-1 antibody is directed to the platelet's activated
IIbIIIa (CD62) cell adhesion molecule (CAM). This is the same molecule
which binds fibrinogen and thus is probably the reason you are seeing
competitive interference.
/Dan
----------
{From: owner-cyto-sendout
{To: Cytometry Mailing List
{Subject: Competition between fibrinogen and PAC-1
{Date: Tuesday, January 06, 1998 6:51AM
{
{
{Dorte,
{ You say you are using a whole blood medium for your assays - what=
{
{anticoagulant are you using ?
{
{My experience with PLT antibodies is that some, but not all, are very
{unpredictable if EDTA is used although I'm not sure what CD specificity
{PAC-1 is - I don't have my charts with me.
{
{
{Wal Sharp
{
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