Maryalice (and others): My one line dismissal of CD43 was perhaps over-interpreted. I was really thinking of acute leukemias. In particular, we recently had a pediatric autopsy with an acute leukemia-like disease (diagnosed at autopsy) that was a "CD43 only" phenotype in paraffin and frozen tissue after performing about two dozen markers. In addition, T-cell and B-cell antigen receptors were germline. I do believe that there is some utility for CD43 in combination with other markers in distinguishing low grade B-cell malignancies, as has been pointed out. Have a Happy Holiday. Howard On Fri, 19 Dec 1997, Maryalice Stetler-Stevenson wrote: > > Howard, > I have to disagree with you. Few markers are lineage specific. TDT > can be found in ALL and AML but it is still useful. I find CD43 to be > useful when evaluated in a panel of antibodies. CD43 positivity by a B-cell > monomorphic process is very useful. CD43 positivity alone is worthless (but > then so is CD20 positivity alone). I think that CD43 is used for IPox > studies because it works well in paraffin sections. However, other > antibodies, that so far only work well on fresh tissue, are more useful. > Therefore we don't use CD43 for flow but do in IPox. > > Maryalice > > > > >CD43 is not lineage specific and therefore has limited diagnostic utility. > > > >The following reference may be of interest to you: > > > >The "CD43 only" phenotype. An aberrant, nonspecific immunophenotype > >requiring comprehensive analysis for lineage resolution. Segal GH et al. > >Am J Clin Pathol 97:861-865, 1992 > > > >Sincerely, > > > >Howard Ratech, MD > > > >On Wed, 17 Dec 1997, Adrian O Vladutiu wrote: > > > >> > >> While antibody to CD43 are being commonly used in immunohistochemistry, > >> for flow cytometry the use of this antibody is not listed in the many > >> consensus reports on antibodies to be used for analysis of lymphoma by > >> flow cytometry. Are there explanations for this? > >> > >> > >> > > Maryalice Stetler-Stevenson > Director Flow Cytometry Unit > Laboratory of Pathology, NCI, NIH > > >
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:27 EST