Howard,
I have to disagree with you. Few markers are lineage specific. TDT
can be found in ALL and AML but it is still useful. I find CD43 to be
useful when evaluated in a panel of antibodies. CD43 positivity by a B-cell
monomorphic process is very useful. CD43 positivity alone is worthless (but
then so is CD20 positivity alone). I think that CD43 is used for IPox
studies because it works well in paraffin sections. However, other
antibodies, that so far only work well on fresh tissue, are more useful.
Therefore we don't use CD43 for flow but do in IPox.
Maryalice
>
>CD43 is not lineage specific and therefore has limited diagnostic utility.
>
>The following reference may be of interest to you:
>
>The "CD43 only" phenotype. An aberrant, nonspecific immunophenotype
>requiring comprehensive analysis for lineage resolution. Segal GH et al.
>Am J Clin Pathol 97:861-865, 1992
>
>Sincerely,
>
>Howard Ratech, MD
>
>On Wed, 17 Dec 1997, Adrian O Vladutiu wrote:
>
>>
>> While antibody to CD43 are being commonly used in immunohistochemistry,
>> for flow cytometry the use of this antibody is not listed in the many
>> consensus reports on antibodies to be used for analysis of lymphoma by
>> flow cytometry. Are there explanations for this?
>>
>>
>>
Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH
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