Dear Maryalice
I agree with you about the utility of CD43 to the point where i do include
it with our examination of B cell tumors. We find it generally helpful in
conjunction with other markers in distinguishing mantle cell tumors from
other low grade lymphomas which tend not to express it.
regards
frederic preffer
At 09:58 AM 12/19/97 -0400, you wrote:
>
>Howard,
> I have to disagree with you. Few markers are lineage specific. TDT
>can be found in ALL and AML but it is still useful. I find CD43 to be
>useful when evaluated in a panel of antibodies. CD43 positivity by a B-cell
>monomorphic process is very useful. CD43 positivity alone is worthless (but
>then so is CD20 positivity alone). I think that CD43 is used for IPox
>studies because it works well in paraffin sections. However, other
>antibodies, that so far only work well on fresh tissue, are more useful.
>Therefore we don't use CD43 for flow but do in IPox.
>
> Maryalice
>
>>
>>CD43 is not lineage specific and therefore has limited diagnostic utility.
>>
>>The following reference may be of interest to you:
>>
>>The "CD43 only" phenotype. An aberrant, nonspecific immunophenotype
>>requiring comprehensive analysis for lineage resolution. Segal GH et al.
>>Am J Clin Pathol 97:861-865, 1992
>>
>>Sincerely,
>>
>>Howard Ratech, MD
>>
>>On Wed, 17 Dec 1997, Adrian O Vladutiu wrote:
>>
>>>
>>> While antibody to CD43 are being commonly used in immunohistochemistry,
>>> for flow cytometry the use of this antibody is not listed in the many
>>> consensus reports on antibodies to be used for analysis of lymphoma by
>>> flow cytometry. Are there explanations for this?
>>>
>>>
>>>
>
>Maryalice Stetler-Stevenson
>Director Flow Cytometry Unit
>Laboratory of Pathology, NCI, NIH
>
>
>
>
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Frederic I. Preffer
preffer@helix.mgh.harvard.edu
Department of Pathology- Warren 525A
100 Blossom St
Massachusetts General Hospital
Boston MA 02114
v(617) 726-7481 fax (617) 724-3164
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