Thanks to everyone who posted a responese to my initial question on CD4/8 double postive staining in a female with hypothyroid. I'd just like to update everyone. Turns out that it was a plasma artifact causing the double labeling. Washing the cells removed the double positive artifact completely. Now I have another question. In 3 of the responses I received they also saw this staining artifact in people on synthetic thyroid hormone for hypothyroid. This seems kind of odd (at least to me) for 2 relatively rare events to coincide like this. I checked the literature but unfortunately flow panels are not routinely run on people with hypothyroid. If anyone has any ideas on this possible(?) correlation please let me know. This is not a pressing issue, just one of those little questions I like to play with. Thanks again, Mike Koratich USARIEM mkoratich@natick-ccmail.army.mil
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