Here's a good one (maybe) . . . Jurkat cells, loaded with indo, hit with ionomycin (1ug/ml). The ratio inverts showing good ca++ flux, yet after a minute or so, the ratio returns to baseline. So, what does it mean? Is the ionomycin concentration perhaps too high, and therefore toxic, thus causing cell death? What do you think? Also . . . same cells, transfected with whatever, but co-transfected to be neomycin resistant . . . under above conditions (indo loaded, ionomycin treated)the ratio moves in the unbound direction, exxentially backwards from expected. Could this be due to the resistance, and a loading issue? Any thoughts will be appreciated. MAK. --- Mark A. KuKuruga, Managing Director University of Michigan Flow Cytometry kukuruga@medmail.med.umich.edu
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