Annexin V and fixation

From: RJasman@ICOS.com
Date: Wed Dec 17 1997 - 14:18:59 EST

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For you gurus of apoptosis:

I have a client that uses Annexin V to monitor apoptosis in mouse cell
lines currently.  He has expressed an interest in moving to human cells
with his experiments.  Our current SOP for human cells is that they must
be fixed to analyze by flow.  He has tried fixation after Annexin V
staining in the past and found that the fixation destroyed the signal
(binding?) of the annexin.

Has anyone been successful in fixing cells after annexin V staining? Do
you have a method?

Thanks,

Rick Jasman
ICOS Corporation

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