Problem with PKH26 labelling of cells is that in the process you lose anywhere from 20-50% or more of the cells you are trying to label and/or they generally get very "Sticky and clumpy" in the process. Also there seems to be a somewhat stimulating action, in that the doubling time for cell populations is speeded up. The cells are also very permeable immediately after the PKH26 labelling as evidenced by the uptake of Trypan blue by about 99% of cells like CEM, or PBMC's, however these then take off quite nicely in tissue culture media or in animal models. The PKH26 labelling process, at least initially, gives you a really different animal. I've never pursued how long it really takes these cells to recover to a more native state, as we've used them primarily for in vivo cell tracking in the PBMC-SCID-HU mouse model.
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