A number of vital stains bind to cytoplasmic protein and may be more suitable for your application. Some emit in the green region (CFDA, SE C1157) or in the red region (SNARF-1 C-6826 or CellTracker orange C-2927). I have only had experience with CFDA, SE which I have used to track cell division. About 1/10 of the dye binds to intracellular protein whilst most of the unbound dye leaks out in the first 24 hours, with very little leaking of stain thereafter. See British J. of Haematology 1997; 98:528-539 for details. Robert Nordon Post Doctoral Fellow Graduate School of Biomedical Engineering University of New South Wales Sydney, 2052 Tel. no. 61-2-9385-3906 Fax 61-2-9663-2108
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