I'd like to thank all those that have responded to my question. I have alittle more info for you. The subject is a human female in her 30s. She is one of our labtechs that we draw when we are playing with new assays. She is in excellent health with the exception of being hypothyroid and on synthetic thyroid hormone. I did CD3/4/8 staining on her and 2 others at the same time. The other 2 where completely normal. She had no normal CD8+ 4- cells. All the CD8+ cells were CD4+. Her CD4+/8- cells looked fine. They had no CD8 expression. The FS/SS profile of the CD8/4 double positives looked normal, and were evenly distributed just like the CD4 cells. The CD45/14 was also good. I am going to run her again next week and include Coulter count. Any further advice very welcome. Thanks, Mike Koratich USARIEM mkoratich@natick-ccmail.army.mil
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