Apoptosis by PI staining?

From: Darren Hickerson (dhickerson@brody.med.ecu.edu)
Date: Thu Dec 04 1997 - 17:05:35 EST


A lab here is trying to identify apoptotic cells by looking at a peak
to the "left" of G1 on FL2A (PI staining).  Does anyone have a
reference that can verify this approach, and how this "lower"
population is supposed to relate to the scatter plot?  Please e-mail
references or give advice (the stuff looks like background noise to
me).  If this has been discussed on this list in the past, please
tell me approximately when and and who I will simply go to the
archives.  Thanks in advance.

Darren Hickerson

dhickerson@brody.med.ecu.edu

Core Flow Cytometry Facility, Brody 4W37
Department of Microbiology and Immunology
East Carolina University School of Medicine
Greenville, NC 27858

Phone: (919) 816-2799
Fax: (919) 816-3021



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