beam spot intensities

From: Kent Cavender-Bares (bares@MIT.EDU)
Date: Thu Dec 04 1997 - 15:41:52 EST


Hi All,

        I am trying to figure out the laser spot intensity used by some
investigators in a paper, an effort hampered by the fact that some of the
details are missing from their methods.  Ultimately, I need to determine an
approximate intensity of the excitation beam for the following two instruments:

(1) a laser-based "FCS" made by Jasco in Tokyo (400 mW at 488 nm)
(2) a FACS Analyzer by BD (mercury-cadmium arc lamp at 485 nm)

        For the FCS system, I need to figure out if there is a "standard"
optics configuration so that I can determine the spot size, and for the FACS
Analyzer, I need both an estimate of the lamp intensity and a description of
the optical configuration.


Any help would be greatly appreciated!

Kent



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