Thanks to many of you who wrote last month about a user here who was getting only about 10% of the total number of cells by flow that she was putting in the tube. To make a long story short, your responses helped me to convince her that her cell count was either wrong or changed during the procedure; furthermore, if final count was important, she should count the cells at many points during the procedure to find out where they are being lost (spinning, permeabilizing, staining, etc.), not just at the very beginning before any manipulation and assume they'd be the same at the end. Another victory for the flow bulletin board. God bless America. Thanks, Darren Hickerson dhickerson@brody.med.ecu.edu Core Flow Cytometry Facility, Brody 4W37 Department of Microbiology and Immunology East Carolina University School of Medicine Greenville, NC 27858 Phone: (919) 816-2799 Fax: (919) 816-3021
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