Dear Ken,
We have seen changes in immunophenotype of follicular lymphoma with
transformation. We have seen development of TDT expression (a couple of
cases) and even switching of light chain from kappa to lambda. We have seen
differences in expression of CD19 and CD22 from one set of lymph nodes to
another at autopsy and, although I haven't seen CD13 in follicular
lymphoma, I have seen it late (ie with progression)in other B-cell
neoplasms. I can believe that these are the same clones. Can you get PCR
done for the t(14;18) breakpoint on the two sites? If the same size PCR
product is seen with both you have your answer.
Maryalice
>A 55 year old female had a history of follicular low grade lymphoma in
>1989. She now presents with ascitis and abdominal nodes. The cells
>show a similar picture: a mixture of small cleaved and predominantly
>large cells. The large cells have characteristics of centrocytes or
>centroblasts. Many have abundant cytoplasm which in giemsa stain is
>blue with vacuoles. There sre also a few giant bizarre multinucleated
>ones. They are proliferative and express p53 strongly.
>
>Phenotypically the lymph node is CD5-, CD10+/20+, CD19weak, CD22+,
>CD23-, monoclonal Kappa strong, I3 strong. T cell markers are
>negative. The ascitic fluid(many very large cells) demonstrates much
>the same except that CD5 is definitely coexpressed with CD20 and CD23 is
>weak fluorescence.
>
>Does this represent the same clone? Does expression of CD5 depend upon
>geographical environment? Any suggestions??
>
>I sent this earlier but had no responses. Hopefully someone will be
>able to comment on this phenotypic presentation. Thank you.
>--
>Dr. Ken Orr, Head Immunology
>St. Boniface General Hospital
>409 Tache Avenue, Winnipeg, Manitoba R2H 2A6
>Tel: (204) 237-2482 Fax: (204) 235-3423
Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:23 EST