On Sun, 30 Nov 1997 05:40:27 -0600 (CST), Hector Bauson Pulido_ Jr. wrote... >I am interested in cryopreserving cells for both research and quality >control purposes. Does anyone know a good method to preserve cells >with minimal cell loss and high viability after thawing? Also, what >equipment should one have for this procedure? > One widely used approach, giving very satisfactory results, is to cryopreserve in DMSO (final concentration 10%), albumin (final concentration 10%), and tissue culture medium. One simply makes up a solution of 20% DMSO, 20% albumin in culture medium, and mixes it with an equal volume of your cell suspension. Programmed-rate freezing is still used in many labs, when the cryopreserved cells are eventually to be transplanted, but most cells tolerate cryopreservation well without it. Just freeze slowly, and thaw rapidly. Cells can be stored in a -80oC freezer (or colder), or in liquid nitrogen. Best of luck. Scott ----------------------------------------------- Scott R. Burger, M.D. Medical Director, Cell Therapy Clinical Laboratory Department of Laboratory Medicine and Pathology University of Minnesota burge009@gold.tc.umn.edu 612-626-4919 612-624-5411 (Fax)
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