The method is my chapter in Current Protocols in Cytometry by John Wiley & Sons. An early draft (with zippy color plots rather than dull black and white ones) is present at: http://nucleus.immunol.washington.edu/Research_facilities/Apps/7aad.html When using PE with 7-AAD, pay close attention to compensation of PE-bright cells vs.. 7-AAD fluorescence. Viable cells could be gated out if compensation is done poorly. Dave Coder dcoder@u.washington.edu -----Original Message----- From: Jan Nelson <j.nelson@auckland.ac.nz> To: cyto-inbox Date: Monday, November 24, 1997 4:06 PM Subject: Viability staining with 7AAD > >Dear Flow Colleagues, >We are seeking a protocol and source of 7AAD (I see Sigma produces one) for >a viability assay. We want to include this in our double staining method >for CD34-PE/CD45-FITC using the ISHAGE protocol. >Any advice about interpretaion would also be appreciated. >many thanks >Jan > >Jan Nelson >Molecular Haematology >Department of Molecular Medicine >The University of Auckland >Phone: (+64)(9) 373-7599 ext 6381 >Fax: (+64)(9) 373-7292 > >
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:22 EST