Jan, Check out: http://nucleus.immunol.washington.edu/Research_facilities/Apps/7aad.html for info about using 7-AAD as a viability indicator. Barry >X-Sender: mmm_jnelson@mednov1 >Mime-Version: 1.0 >Date: Mon, 24 Nov 1997 17:26:35 +1200 >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >From: Jan Nelson <j.nelson@auckland.ac.nz> >Subject: Viability staining with 7AAD > > >Dear Flow Colleagues, >We are seeking a protocol and source of 7AAD (I see Sigma produces one) for >a viability assay. We want to include this in our double staining method >for CD34-PE/CD45-FITC using the ISHAGE protocol. >Any advice about interpretaion would also be appreciated. >many thanks >Jan > >Jan Nelson >Molecular Haematology >Department of Molecular Medicine >The University of Auckland >Phone: (+64)(9) 373-7599 ext 6381 >Fax: (+64)(9) 373-7292 > Barry Grimes FACS Lab Manager Hematopoiesis Center Markey Cancer Center 800 Rose St. cc418 Lexington,KY 40536-0093 lab) 606-323-8193 fax) 606-257-7715 e-mail) bagrim1@pop.uky.edu
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