I need to sort triploid oyster embryos from diploid and then grow the triploids post sorting. We have used propidium iodide staining to confirm that we can induce triploid embryos but PI obviously affects long term viability. Any thoughts would be appreciated. At present we have a BD Vantage with a 100MW aircooled argon laser. I guess this rules out using Hoechst 33342? Cheers Ken Dr. Kenneth W. Beagley Discipline of Pathology Faculty of Medicine and Health Sciences University of Newcastle Callaghan,NSW 2308 Australia TEL 61-02-4923 6157 FAX 61-02-4923 6998 Email mdkwb@mail.newcastle.edu.au "Life is like a dogsled team. If you are not the lead dog the view never changes"
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