Iona,
You might want to try a Stomacher (Tekmar-Dohrmann; Cincinnati, OH
513/247-7000)for your plant tissues. I was able to get suitable
samples from potato leaf tissue for DNA analysis, although it was only
a couple of experiments.
Cindy Gross
Monsanto
cindy.j0.gross@monsanto.com
______________________________ Reply Separator _________________________________
Subject: Dako Medimachine
Author: owner-cyto-sendout@flowcyt.cyto.purdue.edu at INTERNET
Date: 11/19/97 6:07 PM
Hi,
I have recently obtained a Dako medimachine to isolate nuclei
from plant leaf tissue. Right now staring me in the face are 500
hops and 1200 kiwifruit samples and all I am getting is very high
levels of debris and no distinct nuclear peak. If it can be avoided
I do not wish to have to prepare by hand (which is what I have been
doing for the last 7 years).
I have so far tested varying chopping times, the amount of tissue and
micron size.
Does anyone have any suggestions on how to obtain good results with
the medimachine or has tried it on plant tissue?
Please any suggestions would be appreciated,
Thanks
Iona O'Brien.
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