Hi, I have recently obtained a Dako medimachine to isolate nuclei from plant leaf tissue. Right now staring me in the face are 500 hops and 1200 kiwifruit samples and all I am getting is very high levels of debris and no distinct nuclear peak. If it can be avoided I do not wish to have to prepare by hand (which is what I have been doing for the last 7 years). I have so far tested varying chopping times, the amount of tissue and micron size. Does anyone have any suggestions on how to obtain good results with the medimachine or has tried it on plant tissue? Please any suggestions would be appreciated, Thanks Iona O'Brien.
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