Dear Bob, Sorry to be so vague in my previous message. >>What antigens do you assay for? Cytokines by sandwich immunoassay (avidin coated beads; Capture: biotinylated monoclonal, Detection:Bodipy polyclonal) The list: IL-1a,IL-1b,IL-1RA,IL-2,IL-6,IL-8,IL-12,IFNg and TNFa. >>What quantity of antigen is on the beads (as/mg of beads)? Luminex prepared the beads so they should comment on the bead loading >>Are your results quantitative, or just +ve or -ve? Quantitative; all the analyses produced curves with r2>0.99 with the exception of IL-1b. IL-1b showed no binding to the beads at all (reagents?). >>What is the assay detection limit for each antigen? I didn't calculate them, but it appeared to be 2-3 fold higher than our kit ELISA limits. This experiment was a first attempt and not a definitive method validation study; I do not want to draw too many conclusions from it. Kerry Oliver at Luminex had only recently put this panel of cytokines together and had not run anything but standards. However, we considered it enough of a success to keep going. Regards, Gregg ridder.gm@pg.com Phone: (513) 627-2634 FAX: (513) 627-1760 >>Dear Gregg, >>What antigens do you assay for? >>What quantity of antigen is on the beads (as/mg of beads)? >>Are your results quantitative, or just +ve or -ve? >>What is the assay detection limit for each antigen? >>Just a small sample of qq that would be useful. >>Thanks, >>Bob
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